Agnes Tilley was baptized on 7 November 1585 at Henlow, co. Bedford, England, the daughter of Edmund and Mary (Wyne) Cooper. On 20 June 1614, she married Edward Tilley. They moved to Leiden, Holland by 1618, and came on the Mayflower to Plymouth in 1620 with Henry Samson and Humility Cooper. Agnes' sister Martha was the mother of Henry Samson, and her brother Robert was the father of Humility Cooper. Some of the lines of Agnes' ancestry have been traced as far back as Wido de Reinbudcourt, the Doomsday Lord of Chipping Warden and Burton, co. Northampton, who lived in the 11th century, but no royal line has yet been found. Agnes died sometime the first winter at Plymouth, between January and March 1621. She and Edward had no known children.